Caspase-3 Polyclonal Antibody

BTL5857T

Description

Catalog No.:BTL5857T
Applications:IHC-p,ELISA
Reactivity:Human,Rat,Mouse
Gene Name:CASP3 CPP32
Protein Name:Caspase-3
Human Gene Id:836
Human Swiss Prot No:P42574
Mouse Gene Id:12367
Mouse Swiss Prot No:P70677
Rat Swiss Prot No:P55213
Immunogen:Synthetic peptide from human protein at AA range: 134-205
Specificity:The antibody detects endogenous Caspase-3
Formulation:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source:Rabbit
Dilution:IHC-p 1:50-200, ELISA 1:10000-20000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:1 mg/ml
Storage Stability:-20°C/1 year
Other Name:Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]
Background:CASP3 (caspase 3) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apo
Function:regulation of cyclin-dependent protein kinase activity, DNA catabolic process, endonucleolytic, leuk

Products Images

Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:200

References