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Integrin β3 Polyclonal Antibody

BTL5592T
Data Sheet

Description

Catalog No.：BTL5592T
Applications：WB,IHC-p,ELISA
Reactivity：Human
Gene Name：ITGB3
Protein Name：Integrin beta-3
Human Gene Id：3690
Human Swiss Prot No：P05106
Mouse Swiss Prot No：O54890
Immunogen：The antiserum was produced against synthesized peptide derived from the C-terminal region of human ITGB3. AA range:621-670
Specificity：Integrin β3 Polyclonal Antibody detects endogenous levels of Integrin β3 protein.
Formulation：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source：Rabbit
Dilution：WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
Purification：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration：1 mg/ml
Storage Stability：-20°C/1 year
Other Name：ITGB3; GP3A; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61
MolecularWeight(Da)：87214
Observed Band(KD)：87
Background：The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners
Function：cell-substrate junction assembly, cell adhesion, cell-matrix adhesion, cell surface receptor linked

Products Images

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Integrin β3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Integrin β3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Integrin β3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,Integrin β3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Integrin β3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-kidney-cancer tissue. 1,Integrin β3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Western Blot analysis of NIH-3T3 cells using Integrin β3 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#：BTL0002) was diluted at 1:20000
Western Blot analysis of 3T3 cells using Integrin β3 Polyclonal Antibody diluted at 1：2000. Secondary antibody(catalog#：BTL0002) was diluted at 1:20000
Western Blot analysis of hela MCF-7 U2OS 3T3-UV Hela-UV using Integrin β3 Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#：BTL0002) was diluted at 1:20000
Western Blot analysis of 3T3-UV KB-UV mouse-lung mouse-brain 293T using Integrin β3 Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#：BTL0002) was diluted at 1:20000

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