|The antibody was obtained by affinity purified technique, which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. Goat anti-rabbit IgG was conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.
|The antibody conjugate is specific for rabbit IgG. No cross-reactivity was observed with rabbit IgA, IgM proteins by ELISA.
|The antiserum was developed in goat using the rabbit IgG as the immunogen
|ELISA WB IHC
HRP-labeled Goat IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol.
|Store at -20 ℃/1 year
|ELISA : 1:5000~1:20000
Western blotting: 1:5000~1:10000
|Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).