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α-Smooth Muscle Actin Monoclonal antibody

BTL3365M
Data Sheet

Description

Catalog No.：BTL3365M
Applications：WB,IF,IHC-p
Reactivity：Human,Mouse,Rat
Gene Name：ACTA2
Protein Name：Actin, aortic smooth muscle
Human Gene Id：59
Human Swiss Prot No：P62736
Mouse Swiss Prot No：P62737
Rat Swiss Prot No：P62738
Immunogen：Synthetic Peptide of α-SMA
Specificity：The antibody detects endogenous α-SMA protein.
Formulation：PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source：Mouse
Dilution：WB: 1:10000-100000 IF 1:200 IHC:1:1000-2000
Purification：The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage Stability：-20°C/1 year
Other Name：ACTA2; ACTSA; ACTVS; GIG46; Actin, aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Observed Band(KD)：42
Background：The protein encoded by ACTA2 (actin, alpha 2, smooth muscle, aorta) belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integr
Function：muscle system process, circulatory system process, vascular process in circulatory system, muscle co

Products Images

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,α-SMA Monoclonal Antibody(6A12) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,α-SMA Monoclonal Antibody(6A12) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,α-SMA Monoclonal Antibody(6A12) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-liver tissue. 1,α-SMA Monoclonal Antibody(6A12)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Mouse-liver tissue. 1,α-SMA Monoclonal Antibody(6A12)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Rat-liver tissue. 1,α-SMA Monoclonal Antibody(6A12)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain using α-SMA Monoclonal Antibody.
Immunohistochemical analysis of paraffin-embedded Mouse Cecal Tissue using α-SMA Monoclonal Antibody.
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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