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Active Caspase 3 Monoclonal antibody

BTL3431M
Data Sheet

Description

Catalog No.：BTL3431M
Applications：IF,WB,IHC-p
Reactivity：Human,Mouse,Rat,Chicken
Gene Name：CASP3
Protein Name：Caspase-3
Human Gene Id：836
Human Swiss Prot No：P42574
Mouse Swiss Prot No：P70677
Rat Swiss Prot No：P55213
Immunogen：Recombinant Protein of Active Caspase-3
Specificity：The antibody detects endogenous Active Caspase-3 protein.
Formulation：PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Source：Mouse
Dilution：IF: 1:50-200 WB: 1:500-1000 IHC: 1:100-200
Purification：The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage Stability：-20°C/1 year
Other Name：CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Observed Band(KD)：17
Background：CASP3 (caspase 3) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apo
Function：regulation of cyclin-dependent protein kinase activity, DNA catabolic process, endonucleolytic, leuk

Products Images

Immunofluorescence analysis of rat-lung tissue. 1,Active Caspase-3 Monoclonal Antibody(5E1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Active Caspase-3 Monoclonal Antibody(5E1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,Active Caspase-3 Monoclonal Antibody(5E1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,Active Caspase-3 Monoclonal Antibody(5E1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using Active Caspase-3 Monoclonal Antibody.
Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using Active Caspase-3 Monoclonal Antibody.
Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000

References